Dr. Joanna Miest

Scientist

Research Division 3: Marine Ecology
Evolutionary Ecology of Marine Fishes

Office:
Room: A 53
Phone: +49-431 600 4569
Fax: +49-431 600 4553
E-Mail: jmiest(at)geomar.de

Address:
Helmholtz Centre for Ocean Research Kiel (GEOMAR)
Düsternbrooker Weg 20
D-24105 Kiel
Germany

 

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Projects

My research focusses on the development of innovative solutions for increasing productivity in aquaculture, especially focussing on welfare and diseases in aquaculture. Currently I am foccusing on various aspects of turbot immunology and disease detection:

  • Increasing survival in turbot larvae
    In nature but also in the fish prodcution industry the larvae stage of fish is the most sensitive stage with up to 99% of loss.This loss if often associated with disease as the immune system of fish larvae is not fully developed.
    In this project, which is conducted in collaboration with Dr. Carmen Arndt and industrial partners, we are investigating, if the administration of immunomodulants and other feed supplements can increase survival and immunity of turbot larvae.
  • Developing novel non-invasive methods to detect disease
    Infectious diseases are a major concern for this vital food-producing industry, costing $3 billion annually, and threatening the economic sustainability of production (FAO 2012). Diagnosis is especially difficult in aquaculture and infections are often not detected before serious symptoms and death occurs. In addition, current standard diagnostic tools (ELISAs, PCR) only test for the presence of previously described pathogens, are most often invasive, and are limited in that they can only detect infection in each individual tested, and not the population as a whole.
    Addressing this problem I work with Dr. Daniel Bray I am working on a non-invasive method using to detect stress and disease in the surrounding water. This project is partly funded by the DAAD.
  • Effect of stress on skin immunity
    Farming of fish in high densities leads to stress and immunosuppression in the animals. In collaboration with Dr. Mikolaj Adamek I aim to elucidate some of the mechanisms behind the effects of stress on immunity. For this purpose we are using cortisol implants to increase cortisol levels in juvenile turbot and analyse immunity and  and barrier function in skin.
    This project will help us understand how stress can lead to higher infection rates in fish. This knowledge can then be used to develop novel treatment and prevention methods.

Research techniques

  • Molecular techniques: gel-electrophoresis, conventional, reverse transcription and real time PCR (Standart and Biomark Fluidigm systems), primer design, sanger sequencing
  • Histology: fluorescent microscopy, apoptosis stainings on single cells and histological slides
  • Cell culture: culturing of primary cell cultures and cell lines
  • Physiology methods: respiration measurements with oxygen optodes (PreSens) in the open and closed system, cortisol extraction from water and cortisol ELISA
  • Chemical ecology methods: solid phase extraction of odours from water
  • Handling, dissection and sample collection of fish and fish larvae

Career, Employment

  • 2012 – current
    Geomar Helmholtz Centre for Ocean Research
     Postdoc for Aquaculture and Fish Diseases (Project FineAqua)
  • 04/08 – 07/08       
    eurelations AG, Zurich, Switzerland
    Management and administration of science projects

Education

  • 2009 – 2012        
    Keele University
    Ph.D. "Apoptosis and its association with immunomodulation and disease in common carp (Cyprinus carpio L.)" within the EU NEMO project
    Supervisors: Prof. Dave Hoole, Prof. Gwyn Williams
  • 2007 – 2008
    Alfred-Wegener-Institute for Polar and Marine Research in Bremerhaven,Germany
    Dissertation Project "Comparison of temperature related oxygen consumption of isolated liver cells in two different populations of cod (Gadus morhua)."
    This work was conducted at the under supervision of Prof. Hans-Otto Pörtner and Dr. Gisela Lannig.
  • 2001 – 2008
    Albert-Ludwigs University Freiburg, Germany
    • Animal Physiology/Neurobiology/Behaviour
    • Cell biology
    • Ecology/Evolution.

Publications

Number of items: 24.

Articles in a Scientific Journal - peer-reviewed

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Miest, J. J., Arndt, C., Adamek, M., Steinhagen, D. and Reusch, T. B. H. (2016) Dietary β-glucan (MacroGard®) enhances survival of first feeding turbot (Scophthalmus maximus) larvae by altering immunity, metabolism and microbiota Fish & Shellfish Immunology, 48 . pp. 94-104. DOI 10.1016/j.fsi.2015.11.013.

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Miest, J. J. and Hoole, D. (2015) Time and concentration dependency of MacroGard® induced apoptosis Fish & Shellfish Immunology, 42 (2). pp. 363-366. DOI 10.1016/j.fsi.2014.11.009.

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Miest, J. J., Adamek, M., Pionnier, N., Harris, S., Matras, M., Rakus, K. L., Irnazarow, I., Steinhagen, D. and Hoole, D. (2015) Differential effects of Alloherpesvirus CyHV-3 and Rhabdovirus SVCV on apoptosis in fish cells Veterinary Microbiology, 176 (1-2). pp. 19-31. DOI 10.1016/j.vetmic.2014.12.012.

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Pionnier, N., Adamek, M., Miest, J. J., Harris, S. J., Matras, M., Rakus, K. Ł., Irnazarow, I. and Hoole, D. (2014) C-reactive protein and complement as acute phase reactants in common carp Cyprinus carpio during CyHV-3 infection Diseases of Aquatic Organisms, 109 (3). pp. 187-199. DOI 10.3354/dao02727.

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Falco, A., Miest, J. J., Pionnier, N., Pietretti, D., Forlenza, M., Wiegertjes, G. F. and Hoole, D. (2014) β-Glucan supplemented diets increase poly(I:C)-induced gene expression of Mx, possibly via Tlr3-mediated recognition mechanism in common carp (Cyprinus carpio) Fish & Shellfish Immunology, 36 (2). pp. 494-502. DOI 10.1016/j.fsi.2013.12.005.

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Miest, J. J., Roth, O. and Reusch, T. B. H. (2013) Linking fish diseases and animal welfare in aquaculture – A study of the turbot herpesvirus Fish & Shellfish Immunology, 34 (6). pp. 1724-1725. DOI 10.1016/j.fsi.2013.03.271.


Pionnier, N., Falco, A., Miest, J. J., Frost, P., Irnazarow, I., Shrive, A. and Hoole, D. (2013) Dietary β-glucan stimulate complement and C-reactive protein acute phase responses in common carp (Cyprinus carpio) during an Aeromonas salmonicida infection Fish & Shellfish Immunology, 34 (3). pp. 819-831. DOI 10.1016/j.fsi.2012.12.017.


Falco, A., Frost, P., Miest, J. J., Pionnier, N., Irnazarow, I. and Hoole, D. (2012) Reduced inflammatory response to Aeromonas salmonicida infection in common carp (Cyprinus carpio L.) fed with β-glucan supplements Fish & Shellfish Immunology, 32 (6). pp. 1051-1057. DOI 10.1016/j.fsi.2012.02.028.


Miest, J. J., Falco, A., Pionnier, N. P. M., Frost, P., Irnazarow, I., Williams, G. T. and Hoole, D. (2012) The influence of dietary β-glucan, PAMP exposure and Aeromonas salmonicida on apoptosis modulation in common carp (Cyprinus carpio) Fish & Shellfish Immunology, 33 (4). pp. 846-856. DOI 10.1016/j.fsi.2012.07.014.

Articles in a Scientific Journal - without review


Falco, A., Miest, J. J., Pionnier, N., Pietretti, D., Forlenza, M., Wiegertjes, G. F. and Hoole, D. (2013) β-glucan supplemented diets induce high and broad expression levels of TLR3 what explains protection conferred by these additives against viral infections in fish Fish & Shellfish Immunology, 34 (6). p. 1706. DOI 10.1016/j.fsi.2013.03.211.


Miest, J. J., Adamek, M., Steinhagen, D. and Hoole, D. (2013) The role of apoptosis in immunomodulation by beta-glucan during in vitro viral infection Fish & Shellfish Immunology, 34 (6). p. 1666. DOI 10.1016/j.fsi.2013.03.100.

Book chapters


Nielsen, M. E., Miest, J. J. and Schmidt, J. G. (2013) The Immune System In: Eel Physiology , ed. by Trischitta, F., Takei, Y. and Sebert, P.. CRC Press, Boca Raton, Fl., USA, p. 298. ISBN 978-1-4665-9827-0

Presentations


Miest, J. J., Adamek, M., Steinhagen, D. and Hoole, D. (2013) The role of apoptosis in immunomodulation by beta-glucan during in vitro viral infection [Talk] In: 1. International Fish & Shellfish Immunology Conference, 25.-28.06.2013, Vigo, Spain .


Miest, J. J., Falco, A., Pionnier, N. P. M., Frost, P., Irnazarow, I. and Hoole, D. (2011) Apoptosis in common carp (Cyprinus carpio) - effect of β-glucan and Aeromonas salmonicida infection [Talk] In: 15. European Association of Fish Pathology Conference : International Conference on Diseases of Fish and Shellfish, 12.-16.09.2011, Split, Croatia .

Conference posters


Miest, J. J. and Arndt, C. (2014) The Effect of Immunomodulation on larval survival and immunity in turbot (Scophthalmus maximus) [Poster] In: Aquaculture Europe Conference, 14.-17.10.2014, San Sebastian, Spain .


Arndt, C., Miest, J. J., Sommer, U. and Ueberschär, B. (2014) Effect of processed copepods as food source on fish larval performance [Poster] In: Aquaculture Europe Conference, 14.-17.10.2014, San Sebastian, Spain .


Miest, J. J. and Arndt, C. (2014) Immunomodulation in turbot larvae and its effect on survival and immunity [Poster] In: 11. International Congress on the biology of fish (ICBF), 03.-07.08.2014, Edinburgh, Scotland .


Miest, J. J. and Reusch, T. B. H. (2014) Stress and skin immunology in turbot [Poster] In: 11. International Congress on the biology of fish (ICBF), 03.-07.08.2014, Edinburgh, Scotland .


Miest, J. J., Roth, O. and Reusch, T. B. H. (2013) Linking fish diseases and animal welfare in aquaculture – A study of the turbot herpesvirus [Poster] In: 1. International Fish & Shellfish Immunology Conference, 25.-28.06.2013, Vigo, Spain .


Falco, A., Miest, J. J., Pionnier, N., Pietretti, D., Forlenza, M., Wiegertjes, G. F. and Hoole, D. (2013) β-glucan supplemented diets induce high and broad expression levels of TLR3 what explains protection conferred by these additives against viral infections in fish [Poster] In: 1. International Fish & Shellfish Immunology Conference, 25.-28.06.2013, Vigo, Spain .


Miest, J. J., Falco, A., Pionnier, N. P. M., Frost, P., Irnazarow, I. and Hoole, D. (2012) Effects of dietary β-glucan and additional bacterial exposure on apoptosis in common carp (Cyprinus carpio) [Poster] In: Prebiotics and probiotics in medicine, veterinary sciences and aquaculture: the future, 10.-11.09.2012, Keele, Staffordshire, UK .


Falco, A., Pionnier, N., Miest, J. J., Cartwright, J. R., Wiegertjes, G. F. and Hoole, D. (2011) C-reactive proteins from common carp (Cyprinus carpio): molecular characterization, expression pattern and polymorphisms [Poster] In: 15. European Association of Fish Pathology Conference : International Conference on Diseases of Fish and Shellfish, 12.-16.09.2011, Split, Croatia .


Miest, J. J., Adamek, M., Steinhagen, D. and Hoole, D. (2011) Effects of spring viremia of carp virus, koi herpes virus, and β-glucan on apoptosis in the carp brain cell line [Poster] In: 15. European Association of Fish Pathology Conference : International Conference on Diseases of Fish and Shellfish, 12.-16.09.2011, Split, Croatia .

This list was generated on Fri Jul 29 14:01:21 2016 CEST.