Left: Green sea urchins (Strongylocentrotus droebachiensis) from the Kattegat at GEOMAR, ca. 4-6 cm in diameter (Photo: F. Melzner); center: In the laboratory fertilized sea urchin embryos, 2 cell stage, ca. 0,1mm in diameter (Photo: M. Stumpp); right: Pluteus larvae raised in the laboratory (ca. 0,2mm long), skeleton (dark, long structures) and stomach/intestine (central) are visible. Pluteus larvae move with the help of fine cilia on their arms and live a few weeks in plankton (Photo: M. Stumpp).

Left: Pluteus larvae (ca. 0,2 mm long) under the confocal microscope (spines marked in red). The larva is held under the microscope with a pipette in a water bath so that the liquid in the water bath is continuously exchanged; right: the same larva with the pH sensitive colorant. The calcifying cells (called PMC cells) are visible as green spots along the spines in the arms (Photos: M. Gutowska, M. Stumpp).

Left: Microscopic view of a sea urchin larva. Spines visible as dark structures; right: same section, calcifying and other cells, as well as the membranes around the spines are coloured green by a special colorant. Pluteus larvae are capable of controlling the pH value within these cells. In the surrounding body fluid, the pH regulation does not work and the pH value follows that of the sea water (Photos: M. Gutowska).

24.10.2012

Acidic Water Impacts Sea Urchins

Kiel study verifies adverse effects on sea urchin larvae

24 October 2012/Kiel. Increasing ocean acidification can have negative effects on calcifying marine organisms. A new study of the GEOMAR Helmholtz Centre for Ocean Research Kiel, Kiel University and the University of Gothenburg demonstrates that sea urchin larvae grow and build up their calcium skeletons more slowly if their development has to take place in acidified sea water. In an article in the online edition of the journal Proceedings of the Na-tional Academy of the Sciences, the scientists point out potential mechanisms of effect. The study was carried out within the scope of the BMBF (Federal Ministry of Education and Re-search) coordinated project BIOACID (Biological Impacts of Ocean Acidification) and the Cluster of Excellence “The Future Ocean”.

Pluteus larvae of the green sea urchin Strongylocentrotus droebachiensis are tiny, about 0.3 mm long “eating machines” which feed on algae for several weeks in plankton before they go through metamorphosis and begin their life on the sea bed. Thin skeletal elements support their transparent bodies. These spines are made of calcium carbonate and are formed by special cells. Only recently has it been discovered that pluteus larvae grow more slowly in acidified sea water and need more energy. An extended development period, however, increases the risk that the larvae will be eaten by the many predators in the open sea.

As part of the BMBF coordinated project BIOACID, scientists from GEOMAR Helmholtz Centre for Ocean Research Kiel and the Cluster of Excellence “The Future Ocean” at Kiel University examined which mechanisms lead to this decrease in growth rate. Using methods developed for research on mammals, the scientists were able to show that pluteus larvae cannot control the pH value in their cavities. These measurements were taken with tiny, 2 µm thin pH-electrodes. In contrast, measurements with pH sensitive colorants revealed that the cells in the bodies of larvae can control their internal pH value under the stress of acidification. This is important as the first skeletal elements are formed inside the cell itself – a process that only functions at very uniform conditions. Changes in the pH gradient between the calcifying cells and the cavities could be one of the reasons for the observed reduction in growth and calcification in an acidified sea environment. Under these conditions, more energy is necessary to control the pH value in the calcifying cells. This energy is thus missing for the growth process.

 

“We need further studies on the cell biology of calcification processes in order to better understand the mechanisms of sensitivity” explains Prof. Dr. Frank Melzner, head of the research group for ecophysiology at GEOMAR. “The pH regulation abilities of tiny larval stages can only be researched with sophisticated optical and electric methods” continues Melzner. “That is why the cooperation with human physiologists at the faculty of medicine is so very important, as they have developed methods over decades that are very useful for us marine biologists.” Prof. Dr. Markus Bleich, director of the Physiological Institute of Kiel University confirms this, “For the first time we can transfer techniques, which we have used successfully on mammals for a long time, to the cell processes of sea urchins. The interdisciplinary research for the coordinated project BIOACID and “The Future Ocean” allows us to examine the disturbances of the inner milieu of the cells of different organisms. Thus we can better understand the physiological processes necessary for vital functions.”

The first co authors of the study, Dr. Meike Stumpp and Dr. Marian Hu, a former PhD student at GEOMAR and a former research assistant at Kiel University, are currently completing one-year postdoc positions at the University of Gothenburg. There they are continuing their research on sea urchin larvae. First results point towards further fundamental findings on the physiology of sea urchin larvae. “These studies show that our understanding of the functioning of early life stages of even the most common sea organisms is still in its infancy – exciting times for interdisciplinary marine research” sums up Prof. Melzner 

Original Paper:

Stumpp, M., Hu, M.Y., Melzner, F., Gutowska, M.A., Dorey, N., Himmerkus, N., Holtmann, W., Dupont, S.T., Thorndyke, M.C., Bleich, M., 2012: Acidified seawater impacts sea urchin larvae pH regulatory systems relevant for calcification. Proceedings of the National Academy of Sciences, DOI: 10.1073/pnas.1209174109.

Links:

BMBF coordinated project “Biological Impacts of Ocean ACIDification" (BIOACID), www.bioacid.de

Cluster of Excellence “The Future Ocean”, www.futureocean.org and www.futureocean.org/evolving-ocean

Contact:

Prof. Dr. Frank Melzner, GEOMAR, fmelzner(at)geomar.de

Prof. Dr. Markus Bleich, CAU Kiel, m.bleich(at)physiologie.uni-kiel.de

Dr. Andreas Villwock, GEOMAR, Communication & Media, Tel.: (0049)431-600-2802, avillwock(at)geomar.de

Friederike Balzereit, Cluster of Excellence “The Future Ocean”, Public Outreach, Tel.: (0049)431-880-3032, fbalzereit(at)uv.uni-kiel.de