METHOD/DESCRIPTION FLUORIDE

APPARATUS

Eppendorfpipettes

Ionselective electrode for fluoride (Orion)

pH-meter with mV-display

Magnetic stirrer

REAGENTS:

TISAB-III-Buffer : 300g Na-Citrate * 2 H2O

                     22g Titriplex IV (1,2-Cyclohexylendinitrilotetra-acetic-acid-Monohydrate)

                     60g NaCl

                     make up to 1000 dm3 with aqua bidest.                    

STANDARDS:

IAPSO-seawater standard (theoretical content of fluorid: 69,5 µM)

Stockstandard: NaF-Standard (52,636 mM) (Merck)

Work standards: Dilute the stock-standard with IAPSO in different relations to obtain standards with different concentrations for a calibration.

Calibration area: 69,5- 174,76 µM/l

PROCEDURE:

1000 µl sample or standard are mixed with 1000 µl TISAB-III-Buffer on the magnetic stirrer. After 5 minutes (value has to be constant!) the result can be read in mV.

  • Head:

    <link mitarbeiter fb2 mg kwallmann _blank einen externen link in einem neuen>Prof. Dr. Klaus Wallmann
    Tel.: 0431-600 2287
    Fax: 0431-600 2928

    Technique /Operator

    <link de mitarbeiter fb2 mg bdomeyer _blank einen externen link in einem neuen>Bettina Domeyer
    Tel.: 0431-600 2230
    Fax: 0431-600 2928

    <link de mitarbeiter fb2 mg ableyer _blank einen externen link in einem neuen>Anke Bleyer
    Tel.: 0431-600 2230
    Fax: 0431-600 2928

    <link typo3 null _blank einen externen link in einem neuen>Regina Surberg
    Tel.: 0431-600 2211
    Fax: 0431-600 2928