Flow cytometry

Flow cytometry is a laser-based technology which is becoming increasingly popular in plankton ecology as it enables automated counting of individual cells, which can be distinguished based on their specific scatter and fluorescence signatures. Flow cytometers consist of a fluidic system where the water sample containing the cells is jacketed by a fast moving fluid in such a way that the cells pass a laser one by one. Light scatter and fluorescence emitted by the cells is recorded in the detector unit and subsequently processed to characterize the cells.
The research unit Biological Oceanography operates an Accuri C6 (BD Biosciences), a Cytosense (Cytobuoy) and two BD FACSCalibur flow cytometer. Both are bench top machines, ideally suited for fieldwork. The Accuri C6 is used primarily to count smaller autotrophs, bacteria, and viruses since the machine has extremely sensitive detectors. The Cytosense is used for larger organisms as it can handle larger volumes. It also takes photographs of individual plankton organisms which greatly facilitates characterization of the counted cells.

Contact: Mathias Hausnost, Jana Meyer, Tania Klüver

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Examples for detection signals from two analysed ciliates and diatom cells in a chain deriving from a seawater sample.

  • Head of Research Unit Biological Oceanography (RD2-BI)

    Prof. Dr. Ulf Riebesell
    GEOMAR Helmholtz Centre for Ocean Research Kiel
    west shore campus, room 20-329
    Düsternbrooker Weg 20, D-24105 Kiel, Germany
    phone: +49 431 600-4444
    e-mail: uriebesell(at)geomar.de

    Personal Assistant/Office Management RD2-BI
    Silvana Gagliardi, room 20-328
    phone: +49 431 600-4445
    fax: +49 431 600-134445
    e-mail: bi-sekr(at)geomar.de

     

    Working group Biogeochemical Processes (RD2-BI/BP)

    Prof. Dr. Ulf Riebesell
    GEOMAR Helmholtz Centre for Ocean Research Kiel
    west shore campus, room 20-329
    Düsternbrooker Weg 20, D-24105 Kiel, Germany
    phone: +49 431 600-4444
    e-mail: uriebesell(at)geomar.de

    Personal Assistant/Office Management RD2-BI/BP
    Silvana Gagliardi, room 20-328
    phone: +49 431 600-4445
    fax: +49 431 600-134445
    e-mail: sgagliardi(at)geomar.de

     

    Working group Microbial Biogeochemistry (RD2-BI/MB)

    Prof. Dr. Anja Engel
    GEOMAR Helmholtz Centre for Ocean Research Kiel
    west shore campus, room 20-025
    Düsternbrooker Weg 20, D-24105 Kiel, Germany
    phone: +49 431 600-1510
    e-mail: aengel(at)geomar.de

    Personal Assistant/Office Management RD2-BI/MB
    Monika Peschke, room 20-021
    phone: +49 431 600-4447
    fax: +49 431 600-4448
    e-mail: mpeschke(at)geomar.de