Monosaccharide analysis

Carbohydrate analysis

Simple carbohydrates are quantified applying high performance ion exchange chromatography, coupled with pulsed amperometric detection (HPAEC-PAD) after Engel and Händel (2011).  Total, dissolved and particulate fractions can be seperated employing different filtration methods. The following carbohydrates can be measured:

Neutral sugars: Fucose, rhamnose, arabinose, galactose, xylose, mannose and glucose.

Amino sugars: Galactosamine, glucosamine.

Acidic sugars: Galacturonic acid, gluconic acid, glucuronic acid and muramic acid.

Prior to analysis the salt has to be removed from the sample using dialysis. In order to quantify the single sugars, the samples are hydrolyzed applying heat and 1M HCl. The acid is removed introducing the sample in a microwave under vacuum and surrounded by a nitrogen atmosphere.

The instrument used for this analysis is a Dionex ICS-3000 equipped with a double pump system and an autosampler.

Reference: Engel, A. und Händel, N. (2011) A novel protocol for determining the concentration and composition of sugars in particulate and in high molecular weight dissolved organic matter (HMW-DOM) in seawater Marine Chemistry, 127 . pp. 180-191. DOI 10.1016/j.marchem.2011.09.004.
 

contact: Prof. Dr. Anja Engel, Jon Roa

  • Head of Research Unit Biological Oceanography (RD2-BI)

    Prof. Dr. Ulf Riebesell
    GEOMAR Helmholtz Centre for Ocean Research Kiel
    west shore campus, room 20-329
    Düsternbrooker Weg 20, D-24105 Kiel, Germany
    phone: +49 431 600-4444
    e-mail: uriebesell(at)geomar.de

    Personal Assistant/Office Management RD2-BI
    Silvana Gagliardi, room 20-328
    phone: +49 431 600-4445
    fax: +49 431 600-134445
    e-mail: bi-sekr(at)geomar.de

     

    Working group Biogeochemical Processes (RD2-BI/BP)

    Prof. Dr. Ulf Riebesell
    GEOMAR Helmholtz Centre for Ocean Research Kiel
    west shore campus, room 20-329
    Düsternbrooker Weg 20, D-24105 Kiel, Germany
    phone: +49 431 600-4444
    e-mail: uriebesell(at)geomar.de

    Personal Assistant/Office Management RD2-BI/BP
    Silvana Gagliardi, room 20-328
    phone: +49 431 600-4445
    fax: +49 431 600-134445
    e-mail: sgagliardi(at)geomar.de

     

    Working group Microbial Biogeochemistry (RD2-BI/MB)

    Prof. Dr. Anja Engel
    GEOMAR Helmholtz Centre for Ocean Research Kiel
    west shore campus, room 20-025
    Düsternbrooker Weg 20, D-24105 Kiel, Germany
    phone: +49 431 600-1510
    e-mail: aengel(at)geomar.de

    Personal Assistant/Office Management RD2-BI/MB
    Monika Peschke, room 20-021
    phone: +49 431 600-4447
    fax: +49 431 600-4448
    e-mail: mpeschke(at)geomar.de