Monosaccharide analysis

Carbohydrate analysis

Simple carbohydrates are quantified applying high performance ion exchange chromatography, coupled with pulsed amperometric detection (HPAEC-PAD) after Engel and Händel (2011). Total, dissolved and particulate fractions can be seperated employing different filtration methods. The following carbohydrates can be measured:

Neutral sugars: Fucose, rhamnose, arabinose, galactose, xylose, mannose and glucose.

Amino sugars: Galactosamine, glucosamine.

Acidic sugars: Galacturonic acid, gluconic acid, glucuronic acid and muramic acid.

Prior to analysis the salt has to be removed from the sample using dialysis. In order to quantify the single sugars, the samples are hydrolyzed applying heat and 1M HCl. The acid is removed introducing the sample in a microwave under vacuum and surrounded by a nitrogen atmosphere.

The instrument used for this analysis is a Dionex ICS-3000 equipped with a double pump system and an autosampler.

Reference: Engel, A. und Händel, N. (2011) A novel protocol for determining the concentration and composition of sugars in particulate and in high molecular weight dissolved organic matter (HMW-DOM) in seawater Marine Chemistry, 127 . pp. 180-191. DOI 10.1016/j.marchem.2011.09.004.

contact: Prof. Dr. Anja Engel, Jon Roa

Example for a chromatogram during an analysis

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Marine Biogeochemistry

Head of the Research Division Biological Oceanography (FB2-BI)
Prof. Dr. Anja Engel
GEOMAR Helmholtz-Zentrum für Ozeanforschung Kiel
Seefischmarkt / building 5, room 5-2.116
Wischhofstr. 1 - 3, 24148 Kiel
Phone:++49/( 0)431 600-1510
email: aengel(at)geomar.de
Administrative Assistant/Department Office FB2-BI
Silvana Gagliardi, room 5-2.310
Phone: ++49 (0)431 600-4445
Telefax: 0431 600-134445
email: bi-sekr(at)geomar.de
W Mikrobielle Biogeochemie (FB2-BI/MB)
Prof. Dr. Anja Engel
GEOMAR Helmholtz-Zentrum für Ozeanforschung Kiel
Seefischmarkt / Gebäude 5, Raum 5-2.116
Wischhofstraße 1 - 3, 24148 Kiel
Telefon: 0431 600-1510
E-Mail: aengel(at)geomar.de
Assistenz/Geschäftszimmer FB2-BI/MB
Monika Peschke, Raum 5-2.310
Telefon: 0431 600-4447
E-Mail: mpeschke(at)geomar.de
Arbeitsgruppe Biogeochemische Prozesse (FB2-BI/BP)
Prof. Dr. Ulf Riebesell
GEOMAR Helmholtz-Zentrum für Ozenforschung Kiel
Seefischmarkt / Gebäude 5, Raum 5-2.313
Wischhofstraße 1 - 3, 24148 Kiel
Telefon: 0431 600-4444
E-Mail: uriebesell(at)geomar.de
Assistenz/Geschäftszimmer FB2-BI/BP
Silvana Gagliardi, Raum 5-2.310
Telefon: 0431 600-4445
Telefax: 0431 600-134445
E-Mail: sgagliardi(at)geomar.de
Arbeitsgruppe Plankton, Biogeochemie und Dynamik (FB2-BI/PBD)
Prof. Dr. Rainer Kiko
GEOMAR Helmholtz-Zentrum für Ozenforschung Kiel
Seefischmarkt / Gebäude 5, Raum 5-4.105
Wischhofstraße 1 - 3, 24148 Kiel
Telefon: 0431 600-1751
E-Mail: rkiko(at)geomar.de
Assistenz/Geschäftszimmer FB2-BI/PBD
Silvana Gagliardi, Raum 5-2.310
Telefon: 0431 600-4445
Telefax: 0431 600-134445
E-Mail: sgagliardi(at)geomar.de
European Research Council Advanced Grant for Prof. Dr. Ulf Riebesell!
Peer-reviewed publications of the research unit Biological Oceanography (since 2004)

Reference: Engel, A. und Händel, N. (2011) A novel protocol for determining the concentration and composition of sugars in particulate and in high molecular weight dissolved organic matter (HMW-DOM) in seawater Marine Chemistry, 127 . pp. 180-191. DOI 10.1016/j.marchem.2011.09.004.

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