My laboratory has a long-term commitment to develop new technologies and procedures towards developing an experimental model for sponge-microbe symbioses. In this context, we have made considerable efforts to increase access and tractability of sponge symbioses. Marine gnotobiotic biosafety S1 animal housing facilities allow experimentation in a germ-free laboratory environment. Experiments are being run to determine the ideal antibiotic cocktail composition and concentration to effectively remove bacteria without causing harm to the sponges. We are further making increasingly use of sponge genomic and transcriptomic resources. Our overarching aim is to move sponge symbioses from an exploratory type of research into the arena of experimental models for host-microbe interactions.
Turon M, Cristina Díez-Vives, Tyler J. Carrier, Laura de la Cruz, Maria Conejero, Sabrina Jung, Lucía Pita, Vasiliki Koutsouveli, Ute Hentschel, Ana Riesgo. Phagocytosis of an obligate symbiont supports embryonic nutrition in the sponge Halichondria panicea. in press
Marulanda-Gomez A, Mueller B, Bayer K, Abukhalaf M, Tholey A, Fraune S, Pita L, Hentschel U (2024) Combined cellular and proteomics approach suggest differential processing of native and foreign vibrios in the sponge Halichondria panicea. mBio bioRxiv preprint doi: https://doi.org/10.1101/2024.11.11.623018
Steiner LX, Schmittmann L, Rahn T, Lachnit T, Jahn MT, Hentschel U (2024) Phage-induced disturbance of a marine sponge microbiome. Environmental Microbiome 19, 97. https://doi.org/10.1186/s40793-024-00637-7
Marulanda-Gomez AM, Ribes M, Franzenburg, Hentschel U and Pita L (2024) Transcriptomic responses of Mediterranean sponges upon encounter with symbiont microbial consortia. BMC genomics, 25(1), 1-13. DOI https://doi.org/10.1186/s12864-024-10548-z
Marulanda-Gomez AM, Bayer K, Pita L, Hentschel U (2023) A novel in-vivo phagocytosis assay to gain cellular insights on sponge-microbe interactions. Front Mar Sci 10. doi: 10.3389/fmars.2023.1176145
Schmittmann L, Rahn T, Busch K, Pita L, Hentschel U (2022) Stability of a dominant sponge-symbiont in spite of antibiotic-induced microbiome disturbance. Environm Microbiol First published: 17 October 2022 https://doi.org/10.1111/1462-2920.16249
Schmittmann L, Jahn M, Pita L, Hentschel U (2020) Decoding cellular dialogues between sponges, bacteria and phages. In Cellular Dialogues in the Holobiont (Bosch & Hadfield, eds.) CRC Press, USA. pp. 49-63. https://doi.org/10.1201/9780429277375
Pita L, Fraune S, Hentschel U (2016) Emerging sponge models of animal-microbe symbioses. Front. Microbiol. doi: 10.3389/fmicb.2016.02102
SFB 1182/2: Origin and Function of Metaorganisms
